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Title

Monte Carlo simulation of multiphoton fluorescence microscopic imaging through inhomogeneous tissuelike turbid media

Author(s)

Deng, Xiaoyuan Deng;  Gan, Xiaosong;  Gu, Min

Abstract

Image resolution and signal level in fluorescence microscopy through inhomogeneous turbid media consisting of scatterers of multiple sizes under single- (1p), two- (2p), and three-photon (3p) excitation have been investigated based on a modified Monte Carlo model. The effects of the size distribution and the concentration distribution of scattering particles are explored. Simulation results reveal that the size and the concentration distribution both have an impact on image formation in media consisting of small particles and that 3p excitation has the most significant impact. In media with scatterers of a large size, both size and concentration distributions lead to a slight effect. Image formation in a mixed medium containing small and large scattering particles is more affected by the large particles.

Publication type

Journal article

Research centre

Swinburne University of Technology. School of Biophysical Science and Electrical Engineering. Centre for Micro-Photonics

Source

Journal of Biomedical Optics, Vol. 8, no. 3 (Jul 2003), pp. 440-449

Publication year

2003

Keyword(s)

Fluorescence;  Biomedical optical imaging;  Monte Carlo methods;  Digital simulation;  Biological tissues;  Image resolution;  Optical microscopy;  Medical image processing

Publisher

SPIE

ISSN

1083-3668

Publisher URL

http://dx.doi.org/10.1117/1.1577116

Copyright

Copyright © 2003 SPIE Society of Photo-Optical Instrumentation Engineers. This paper was originally published in Journal of Biomedical Optics (Vol. 8, no. 3), and is available from: http://dx.doi.org/10.1117/1.1577116. The published version of the paper is reproduced here in accordance with the copyright policy of the publisher. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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