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Title

Three-dimensional optical-transfer-function analysis of fiber-optical two-photon fluorescence microscopy

Author(s)

Bird, Damian;  Gu, Min

Abstract

The three-dimensional optical transfer function is derived for analyzing the imaging performance in fiberoptical two-photon fluorescence microscopy. Two types of fiber-optical geometry are considered: The first involves a single-mode fiber for delivering a laser beam for illumination, and the second is based on the use of a single-mode fiber coupler for both illumination delivery and signal collection. It is found that in the former case the transverse and axial cutoff spatial frequencies of the three-dimensional optical transfer function are the same as those in conventional two-photon fluorescence microscopy without the use of a pinhole. However, the transverse and axial cutoff spatial frequencies in the latter case are 1.7 times as large as those in the former case. Accordingly, this feature leads to an enhanced optical sectioning effect when a fiber coupler is used, which is consistent with our recent experimental observation.

Publication type

Journal article

Research centre

Swinburne University of Technology. School of Biophysical Sciences and Electrical Engineering. Centre for Micro-Photonics

Source

Journal of the Optical Society of America A : Optics, Image Science, and Vision, Vol. 20, no. 5 (May 2003), pp. 941-947

Publication year

2003

Publisher

Optical Society of America

ISSN

1084-7529

Publisher URL

http://www.opticsinfobase.org/abstract.cfm?URI=josaa-20-5-941

Copyright

Copyright © 2003 Optical Society of America. Published version of the paper reproduced here in accordance with the copyright policy of the publisher. This paper was published in the Journal of the Optical Society of America and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://www.opticsinfobase.org/abstract.cfm?URI=josaa-20-5-941. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

Full text

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