Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/1091

Title

Positive and negative tone protein patterning on a photobase generating polymer

Author(s)

Filipponi, Luisa;  Ivanova, Elena P.;  Nicolau, Dan V.;  Pham, Duy K.;  Shirai, M.;  Suyama, K.;  Tsunooka, M.;  Viezzoli, Andrea;  Wright, Jonathon P.

Abstract

This study investigates the use of a copolymer containing O-acryloyl acetophenone oxime (AAPO) and a 4-acryloyloxybenzophenone (ABP) sensitizer as a platform suitable for the patterning of proteins. While low dose UV exposure (312 nm) of the acyloxyimino component photogenerates pendant amine groups at the polymer surface, rendering it protein repelling, the laser ablation method (337 nm) creates hydrophobic features on which proteins adsorb preferentially. These patterning methods produce negative and positive tone protein features, respectively. A critical factor that ensures the quality of the protein patterning is the unusual photodarkening effect for the exposure wavelengths, which concentrates the photoinduced physicochemistry at the very surface of the polymer. The versatility of the proposed patterning methods makes AAPO-ABP an interesting candidate for the patterning of proteins for biochip fabrication.

Publication type

Journal article

Research centre

Swinburne University of Technology. Industrial Research Institute Swinburne

Source

Langmuir, Vol. 19, no. 2 (Dec. 2002), pp. 446-452

Publication year

2003

Publisher

American Chemical Society

Format

pp. 446-452

ISSN

0743-7463

Publisher URL

http://dx.doi.org/10.1021/la026123+

Copyright

© 2003 American Chemical Society.

Peer reviewed