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Title

Polarization anisotropy in fiber-optic second harmonic generation microscopy

Author(s)

Fu, Ling;  Gu, Min

Abstract

We report the investigation and implementation of a compact second harmonic generation microscope that uses a single-mode fiber coupler and a double-clad photonic crystal fiber. Second harmonic polarization anisotropy through the fiber-optic microscope systems is quantitatively measured with KTP microcrystals, fish scale and rat tail tendon. It is demonstrated that the polarized second harmonic signals can be excited and collected through the single-mode fiber coupler to analyze the molecular orientations of structural proteins. It has been discovered that a double-clad photonic crystal fiber can preserve the linear polarization in the core, although a depolarization effect is observed in the inner cladding region. The feasibility of polarization anisotropy measurements in fiber-optic second harmonic generation microscopy will benefit the in vivo study of collagen-related diseases with a compact imaging probe.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics

Source

Optics Express, Vol. 16, no. 7 (Mar 2008), pp. 5000-5006

Publication year

2008

Keyword(s)

Anisotropy;  Compact second harmonic generation microscope;  Computer-assisted image interpretation;  Equipment;  Failure analysis;  Fibre optics;  Harmonic generation;  Image enhancement;  Instrumentation;  Microcrystals;  Microscopes;  Molecular orientations;  Photonic crystals;  Polarization anisotropy;  Polarization microscopy;  Single-mode fiber coupler

Publisher

Optical Society of America

ISSN

1094-4087

Publisher URL

http://dx.doi.org/10.1364/OE.16.005000

Copyright

Copyright © 2008 Optical Society of America. Published version of the paper reproduced here in accordance with the copyright policy of the publisher. This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.16.005000. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.

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