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Title

Vibrio fischeri and Escherichia coli adhesion tendencies towards photolithographically modified nanosmooth poly (tert-butyl methacrylate) polymer surfaces

Author(s)

Ivanova, Elena P.;  Mitik-Dineva, Natasa;  Mocanasu, Radu C.;  Murphy, Sarah;  Wang, James;  Van Reissen, Grant;  Crawford, Russell J.

Abstract

This study reports the adhesion behavior of two bacterial species, Vibrio fischeri and Escherichia coli, to the photoresistant poly(tert-butyl methacrylate) (P(tBMA)) polymer surface. The data has demonstrated that ultraviolet irradiation of P(tBMA) was able to provide control over bacterial adhesion tendencies. Following photolithography, several of the surface characteristics of P(tBMA) were found to be altered. Atomic force microscopy analysis indicated that photolithographically modified P(tBMA) (henceforth termed ‘modified polymer’) appeared as a ‘nanosmooth’ surface with an average surface roughness of 1.6 nm. Although confocal laser scanning microscopy and scanning electron microscopy analysis clearly demonstrated that V. fischeri and E. coli presented largely different patterns of attachment in order to adhere to the same surfaces, both species exhibited a greater adhesion propensity towards the ‘nanosmooth’ surface. The adhesion of both species to the modified polymer surface appeared to be facilitated by an elevated production of extracellular polymeric substances when in contact with the substrate.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Life and Social Sciences

Source

Nanotechnology, Science and Applications, Vol. 1 (Sep 2008), pp. 33-44

Publication year

2008

FOR Code(s)

060106 Cellular Interactions (incl. Adhesion, Matrix, Cell Wall);  100712 Nanoscale Characterisation

Keyword(s)

Bacterial attachment;  Extracellular polymeric substances;  Polymeric surfaces;  Poly(tert-butylmethacrylate);  Surface nanotopography

Publisher

Dove Press

ISSN

1177-8903

Publisher URL

http://www.dovepress.com/articles.php?article_id=2252

Copyright

Copyright © 2008 Ivanova et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.

Full text

Peer reviewed