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- Title
- A 0.4-mm-diameter probe for nonlinear optical imaging
- Author(s)
- Bao, Hongchun; Gu, Min
- Abstract
- A miniaturized probe that possesses a diameter of 0.4 mm is developed for two-photon-excited fluorescence imaging. The miniaturized probe was manufactured by the collapse of air holes and the formation of a lens on the tip of a double-clad photonic crystal fiber (DCPCF) using electric arc discharging from a conventional fusion splicer. As a result, a femtosecond pulsed laser beam delivered by the DCPCF can be directly focused on a sample for two-photon fluorescence imaging. The numerical aperture of the lensed DCPCF is 0.12. The corresponding focal spot size is 6 µm, which is close to the diffraction limit. This 0.4-mm-diamter probe can provide clear two-photon-excited fluorescence images of 10-µm-diameter fluorescent microspheres.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics
- Source
- Optics Express, Vol. 17, no. 12 (2009), pp. 10098-10104
- Publication year
- 2009
- FOR Code(s)
- 0205 Optical Physics
- Keyword(s)
- Endoscopic imaging; Fiber optical imaging; Infrared imaging; Nonlinear microscopy; Three-dimensional image acquisition
- Publisher
- Optical Society of America
- ISSN
- 1094-4087
- Publisher URL
- http://dx.doi.org/10.1364/OE.17.010098
- Copyright
- Copyright © 2009 OSA. Published version of the paper reproduced here in accordance with the copyright policy of the publisher. This paper was published in Optics Express and is made available as an electronic reprint with the permission of OSA. The paper can be found at the following URL on the OSA website: http://dx.doi.org/10.1364/OE.17.010098. Systematic or multiple reproduction or distribution to multiple locations via electronic or other means is prohibited and is subject to penalties under law.
- Full text
- Peer reviewed
