Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/58207

Download PDF (Published version) (Adobe Acrobat PDF, -1 bytes)

Title

Characterization of fibrinogen adsorption onto glass microcapillary surfaces by ELISA

Author(s)

Salim, Malinda;  O'Sullivan, Brian;  McArthur, Sally L.;  Wright, Phillip C.

Abstract

Adsorption of biomolecules onto microchannel surfaces remains a critical issue in microfluidic devices. This paper investigates the adsorption of fibrinogen on glass microcapillaries using an immunoassay method (ELISA) and X-ray photoelectron spectroscopy (XPS). Various adsorption conditions such as protein concentrations and incubation times, buffer pH, buffer ionic strengths and effects of flow are presented. ELISA is successfully demonstrated as a facile and robust technique to examine these phenomena. The highest adsorption level occurs near the isoelectric point of fibrinogen (pH 5.0) and low buffer ionic strengths (0-8 mM). Microchannel surface saturation was achieved at a fibrinogen solution concentration of ~50 µg ml-1. Fibrinogen adsorption under flow was always higher than that seen in static systems. The importance of diffusion phenomena in microchannels on protein adsorption was demonstrated. ELISA experiments using fused silica and PEEK have also confirmed significant adsorption on these mass spectrometer transfer line materials.

Publication type

Journal article

Source

Lab on a Chip: Miniaturisation for Chemistry and Biology, Vol. 7, no. 1 (2007), pp. 64-70

Publication year

2007

FOR Code(s)

03 Chemical Sciences

Keyword(s)

Adsorption;  Buffers;  ELISA;  Enzyme-linked immunosorbent assay;  Fibrinogen;  Hydrogen-ion concentration;  Ionic strength;  Microcirculation;  Microfluidics;  Osmolar concentration;  PEEK;  Photochemistry;  Polyethylene glycols;  Spectrum analysis;  Surface properties;  X ray photoelectron spectroscopy;  XPS

Publisher

Royal Society of Chemistry

ISSN

1473-0197

Publisher URL

http://dx.doi.org/10.1039/b612521m

Copyright

Copyright © 2007 The Royal Society of Chemistry. The authors give the Royal Society of Chemistry the exclusive right and licence throughout the world to edit, adapt, translate, reproduce and publish the Paper in all formats, in all media and by all means (whether now existing or in future devised). Published version of the paper reproduced here for non-commercial purposes only in accordance with the copyright policy of the publisher. This paper is made available for personal use only; no further reuse is permitted.

Full text

Peer reviewed