Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005

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Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/64729

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Title: Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005
Author(s): Swiatek, D. L.; Palombo, E. A.; Lee, A.; Coventry, Michael John; Britz, M. L.; Kirkwood, Carl D.
Abstract: Bovine rotavirus (BRV) has been detected in both dairy and beef cattle herds worldwide. Stool samples collected from calves in the Gippsland region of Victoria, Australia were screened to determine the presence of BRV. A total of 100 faecal samples were collected from calves with and without diarrhoea across three farms during 2004 and 2005. Group A BRV was detected in 26% of faecal samples (22 from diarrheic calves and four from asymptomatic calves). Genotyping analysis of rotavirus positive samples indicated that G6P[5] was the most prevalent genotype (38.5%) followed by G6P[5 + 11] (15.4%). G10P[11] and G6 + G10P[5] were each detected at a rate of 7.7%, and G6 + G10P[11] was found in a single sample (3.8%). Seven samples (26.9%) could not be G and/or P typed. Thirty percent of the BRV positive samples were mixed infections, indicating that individual calves were co-infected with more than one strain of rotavirus. The G6P[5] strains exhibited high VP7 identity (>97% amino acid identity) with B-60, a G6 strain identified in Victorian calves during 1988. A G10P[11] isolate was closely related (>97% amino acid identity in VP7 and VP4 proteins) to a Victorian G10P[11] strain (B-11) also identified during 1988. This study demonstrates that BRV is a contributing pathogen to diarrhoeal disease in Victorian calves, with sequence analysis suggesting long-term conservation of the VP7 protein over a 16-year period.
Publication type: Journal article
Research centre: Swinburne University of Technology. Faculty of Life and Social Sciences. Environment and Biotechnology Centre
Source: Veterinary Microbiology, Vol. 140, no. 1-2 (Jan 2010), pp. 56-62
Publication year: 2010
Keyword(s): Australia; Bovine rotavirus; Calves; Genotyping; Gippsland; Victoria; VP4; VP7
Publisher: Elsevier B.V.
ISSN: 0378-1135
Publisher URL: http://dx.doi.org/10.1016/j.vetmic.2009.07.020
Research Projects: Rotavirus/Calicivirus/Crohn's disease, National Health and Medical Research Council grant number 334364
Peer reviewed