Search Swinburne Research Bank
Home List of Titles Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/64729
- Detection and analysis of bovine rotavirus strains circulating in Australian calves during 2004 and 2005
- Swiatek, D. L.; Palombo, E. A.; Lee, A.; Coventry, Michael John; Britz, M. L.; Kirkwood, Carl D.
- Bovine rotavirus (BRV) has been detected in both dairy and beef cattle herds worldwide. Stool samples collected from calves in the Gippsland region of Victoria, Australia were screened to determine the presence of BRV. A total of 100 faecal samples were collected from calves with and without diarrhoea across three farms during 2004 and 2005. Group A BRV was detected in 26% of faecal samples (22 from diarrheic calves and four from asymptomatic calves). Genotyping analysis of rotavirus positive samples indicated that G6P was the most prevalent genotype (38.5%) followed by G6P[5 + 11] (15.4%). G10P and G6 + G10P were each detected at a rate of 7.7%, and G6 + G10P was found in a single sample (3.8%). Seven samples (26.9%) could not be G and/or P typed. Thirty percent of the BRV positive samples were mixed infections, indicating that individual calves were co-infected with more than one strain of rotavirus. The G6P strains exhibited high VP7 identity (>97% amino acid identity) with B-60, a G6 strain identified in Victorian calves during 1988. A G10P isolate was closely related (>97% amino acid identity in VP7 and VP4 proteins) to a Victorian G10P strain (B-11) also identified during 1988. This study demonstrates that BRV is a contributing pathogen to diarrhoeal disease in Victorian calves, with sequence analysis suggesting long-term conservation of the VP7 protein over a 16-year period.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Faculty of Life and Social Sciences. Environment and Biotechnology Centre
- Veterinary Microbiology, Vol. 140, no. 1-2 (Jan 2010), pp. 56-62
- Publication year
- Australia; Bovine rotavirus; Calves; Genotyping; Gippsland; Victoria; VP4; VP7
- Elsevier B.V.
- Publisher URL
- Copyright © 2009 Elsevier B.V. All rights reserved.
- Research Projects
Rotavirus/Calicivirus/Crohn's disease, National Health and Medical Research Council grant number 334364
- Peer reviewed