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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/72572
- A rapid coupling protocol for the synthesis of peptide nucleic acids
- Vearing, Christopher J.; Fecondo, John V.
- With the current interest in anti-sense and anti-gene technologies, an efficient, fast and less toxic synthesis protocol would be advantageous for the oligomerisation of Peptide Nucleic Acids (PNA). Most of the methods currently in use for the t-Boc synthesis of PNA's use TFA/m-cresol, pyridine, piperidine and capping reagents. In this work, a rapid synthesis protocol has been adapted from an earlier published peptide synthesis method allowing a reduction in cycle time from around 30 min down to 16 min. By utilising quantitative deprotection with 100% TFA, a coupling time of 10 min and a four-fold excess of monomer, this synthesis protocol has been used to synthesise a number of PNA's incorporating all four nucleotides of varying sequence, up to 17 residues in length.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. School of Engineering and Science
- International Journal of Peptide Research and Therapeutics, Vol. 9, no. 4-5 (Jul 2002), pp. 211-219
- Publication year
- FOR Code(s)
- 0601 Biochemistry and Cell Biology; 1101 Medical Biochemistry and Metabolomics
- Antigene oligonucleotides; Antisense oligonucleotides; Peptide nucleic acids; PNA; Solid-phase peptide synthesis; SPPS
- Publisher URL
- Copyright © 2003 Kluwer Academic Publishers.
- Peer reviewed