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Title

Multi-photon fluorescence imaging through biological tissue and image reconstruction

Author(s)

Gan, Xiasong;  Gu, Min

Abstract

In this paper, image formation under single-photon (1-p), two-photon (2-p) and three-photon (3-p) fluorescence imaging through turbid media which consist of different sized scatterers has been investigated in detail. It has been demonstrated that the size of scattering particles plays an important role in determining whether to use 1-p, 2-p, or 3-p excitation. For small scatterers, where Rayleigh scattering is dominant, multi-photon excitation provides significantly better resolution. Such improvement reduces dramatically for large scatterers, where Mie scattering becomes dominant. Another disadvantage of using multi-photon fluorescence excitation in highly scattered media is that penetration depth is limited by fast dropping of signal strength in deep tissue imaging. In this paper, we introduce a deconvolution method with a novel concept of the effective point spread function, which is effective in restoring the loss of imaging resolution caused by multiple scattering in a tissue medium.

Publication type

Conference paper

Research centre

Swinburne University of Technology. School of Biophysical Sciences and Electrical Engineering. Centre for Micro-Photonics

Source

Proceedings of SPIE: Photon Migration, Optical Coherence Tomography, and Microscopy, Munich, Germany, 17 June 2001 / Stefan Andersson-Engels and Michael Kaschke (eds.), Vol. 4431, pp. 226-232

Publication year

2001

Keyword(s)

Deconvolution;  Fluorescence;  Image reconstruction;  Microscopic imaging;  Multi-photon fluorescence;  Photon migration;  Turbid media

Publisher

SPIE

ISSN

0277-786X

Publisher URL

http://dx.doi.org/10.1117/12.447426

Copyright

Copyright © 2003 SPIE Society of Photo-Optical Instrumentation Engineers. This paper was originally published in Proceedings of SPIE (Vol. 4431), and is available from: http://dx.doi.org/10.1117/12.447426. The published version of the paper is reproduced here in accordance with the copyright policy of the publisher. One print or electronic copy may be made for personal use only. Systematic electronic or print reproduction and distribution, duplication of any material in this paper for a fee or for commercial purposes, or modification of the content of the paper are prohibited.

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