Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/74999

Title

Comparison of three-dimensional imaging properties between two-photon and single-photon fluorescence microscopy

Author(s)

Sheppard, C. J. R.;  Gu, Min

Abstract

The imaging performance in single-photon (1-p) and two-photon (2-p) fluorescence microscopy is described. Both confocal and conventional systems are compared in terms of the three-dimensional (3-D) point spread function and the 3-D optical transfer function. Images of fluorescent sharp edges and layers are modelled, giving resolution in transverse and axial directions. A comparison of the imaging properties is also given for a 4Pi confocal system. Confocal 2-p 4Pi fluorescence microscopy gives the best axial resolution in the sense that its 3-D optical transfer function has the strongest response along the axial direction.

Publication type

Journal article

Source

SPIE milestone series: selected papers on multiphoton excitation microscopy / B. R. Masters (ed.), Vol. MS 175 (2003), pp. 127-136

Publication year

2003

Keyword(s)

Confocal microscopy;  Fluorescence microscopy;  Image formation;  Three-dimensional imaging;  Two-photon fluorescence imaging

Publisher

SPIE

ISBN

9780819447487, 081944748X

Publisher URL

http://spie.org/

Copyright

Copyright © 1995 Blackwell Publishing Ltd.

Peer reviewed