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Home List of Titles Comparison of three-dimensional imaging properties between two-photon and single-photon fluorescence microscopy
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/74999
- Comparison of three-dimensional imaging properties between two-photon and single-photon fluorescence microscopy
- Sheppard, C. J. R.; Gu, Min
- The imaging performance in single-photon (1-p) and two-photon (2-p) fluorescence microscopy is described. Both confocal and conventional systems are compared in terms of the three-dimensional (3-D) point spread function and the 3-D optical transfer function. Images of fluorescent sharp edges and layers are modelled, giving resolution in transverse and axial directions. A comparison of the imaging properties is also given for a 4Pi confocal system. Confocal 2-p 4Pi fluorescence microscopy gives the best axial resolution in the sense that its 3-D optical transfer function has the strongest response along the axial direction.
- Publication type
- Journal article
- SPIE milestone series: selected papers on multiphoton excitation microscopy / B. R. Masters (ed.), Vol. MS 175 (2003), pp. 127-136
- Publication year
- Confocal microscopy; Fluorescence microscopy; Image formation; Three-dimensional imaging; Two-photon fluorescence imaging
- 9780819447487, 081944748X
- Publisher URL
- Copyright © 1995 Blackwell Publishing Ltd.
- Peer reviewed