Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/75010

Title: Three-dimensional localisation of fluorescence resonance energy transfer in living cells under two-photon excitation
Author(s): Xu, Ming G.; Crimeen, Blessing; Ludford-Menting, Mandy J.; Gan, Xiaosong; Russell, Sarah; Gu, Min
Abstract: Three-dimensional (3-D) imaging of fluorescence resonance energy transfer (FRET) in human cells under two-photon excitation was demonstrated in this study. A sample was prepared by expressing a donor and an acceptor in living cells and using an antibody to secure the proximity of contact between the donor and the acceptor. The quenching of fluorescence emission of a donor in the double-labelled cell indicates the presence of FRET that occurred in these living cells. Because of the quadratic relation of the excitation power, 3-D localisation of FRET becomes possible.
Publication type: Book chapter
Research centre: Swinburne University of Technology. School of Biophysical Sciences and Electrical Engineering. Centre for Micro-Photonics
Source: SPIE milestone series: selected papers on multiphoton excitation microscopy / B. R. Masters (ed.), pp. 369-373
Publication year: 2003
Keyword(s): 3D microscopy; Fluorescence resonance energy transfer; Micro-spectroscopy; Two-photon microscopy
Publisher: SPIE
ISBN: 9780819447487, 081944748X
Publisher URL: http://spie.org/
Peer reviewed