Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/77232

Title

Impact of nanoscale roughness of titanium thin film surfaces on bacterial retention

Author(s)

Ivanova, Elena P.;  Truong, Vi Khanh;  Wang, James Y.;  Berndt, Christopher C.;  Jones, R. T.;  Yusuf, Iman I.;  Peake, Ian;  Schmidt, Heinrich W.;  Fluke, Christopher;  Barnes, David;  Crawford, Russell J.

Abstract

Two human pathogenic bacteria, Staphylococcus aureus CIP 68.5 and Pseudomonas aeruginosa ATCC 9025, were adsorbed onto surfaces containing Ti thin films of varying thickness to determine the extent to which nanoscale surface roughness influences the extent of bacterial attachment. A magnetron sputter thin film system was used to deposit titanium films with thicknesses of 3, 12, and 150 nm on glass substrata with corresponding surface roughness parameters of Rq 1.6, 1.2, and 0.7 nm (on a 4 μm × 4 μm scanning area). The chemical composition, wettability, and surface architecture of titanium thin films were characterized using X-ray photoelectron spectroscopy, contact angle measurements, atomic force microscopy, three-dimensional interactive visualization, and statistical approximation of the topographic profiles. Investigation of the dynamic evolution of the Ti thin film topographic parameters indicated that three commonly used parameters, Ra, Rq, and Rmax, were insufficient to effectively characterize the nanoscale rough/smooth surfaces. Two additional parameters, Rskw and Rkur, which describe the statistical distributions of roughness character, were found to be useful for evaluating the surface architecture. Analysis of bacterial retention profiles indicated that bacteria responded differently to the surfaces on a scale of less than 1 nm change in the Ra and Rq Ti thin film surface roughness parameters by (i) an increased number of retained cells by a factor of 2−3, and (ii) an elevated level of secretion of extracellular polymeric substances.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Life and Social Sciences

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Industrial Research Institute Swinburne

Research centre

Swinburne University of Technology. Faculty of Information and Communication Technologies. Centre for Astrophysics and Supercomputing

Source

Langmuir, Vol. 26, no. 3 (Feb 2010), pp. 1973-1982

Publication year

2010

Keyword(s)

Bacterial attachment;  Bacterial cells;  Nanoscale roughness;  Pseudomonas aeruginosa;  Staphylococcus aureus;  Surface roughness;  Titanium thin films

Publisher

American Chemical Society

ISSN

0743-7463

Publisher URL

http://dx.doi.org/10.1021/la902623c

Copyright

Copyright © 2009 American Chemical Society. The American Chemical Society does not allow institutions to archive either the accepted manuscript or the published version of the article.

Peer reviewed