Rapid detection of Klebsiella pneumoniae from blood culture bottles by real-time PCR

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/81701

Title: Rapid detection of Klebsiella pneumoniae from blood culture bottles by real-time PCR
Author(s): Kurupati, Prathiba; Chow, Carol; Kumarasinghe, Gamini; Poh, Chit Laa
Abstract: A LightCycler real-time PCR hybridization probe-based assay which detects a partial Klebsiella pneumoniae 16S rRNA gene was developed for the rapid identification of K. pneumoniae directly from growth-positive blood culture bottles (BACTEC 9240 system) within 2 h. No cross-reactivity was observed with 65 negative-control blood cultures that grew bacteria other than K. pneumoniae and 48 negative blood cultures from double-blind experiments, thus demonstrating 100% specificity when compared to results of conventional biochemical characterization. The assay also showed 100% sensitivity, as it correctly identified all 142 positive-control blood cultures and 4 from double-blind trials.
Publication type: Journal article
Source: Journal of Clinical Microbiology, Vol. 42, no. 3 (Mar 2004), pp. 1337-1340
Publication year: 2004
FOR Code(s): 0605 Microbiology; 1108 Medical Microbiology
Keyword(s): Biochemical characterization; Blood culture bottles; Klebsiella pneumoniae; Real-time PCR
Publisher: American Society for Microbiology
ISSN: 1098-660X
Publisher URL: http://dx.doi.org/10.1128/JCM.42.3.1337-1340.2004
Peer reviewed