Home List of Titles Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/81708
- Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis
- Kurupati, Prathiba; Teh, Boon King; Kumarasinghe, Gamini; Poh, Chit Laa
- Klebsiella pneumoniae is an opportunistic pathogen which causes pneumoniae, urinary tract infections and septicemia in immunocompromised patients. Hospital outbreaks of multidrug-resistant K. pneumoniae, especially those in neonatal wards, are often caused by strains producing the extended-spectrum-β-lactamases (ESBLs). An immunoproteome based approach was developed to identify candidate antigens of K. pneumoniae for vaccine development. Sera from patients with acute K. pneumoniae infections (n = 55) and a control group of sera from healthy individuals (n = 15) were analyzed for reactivity by Western blot against ESBL K. pneumoniae outer membrane proteins separated by 2-DE. Twenty highly immunogenic protein spots were identified by immunoproteomic analysis. The immunogenic proteins that are most frequently recognized by positive K. pneumoniae sera were OmpA, OmpK36, FepA, OmpK17, OmpW, Colicin I receptor protein and three novel proteins. Two of the vaccine candidate genes, OmpA (Struve et al. Microbiology 2003, 149, 167-176) and FepA (Lai, Y. C. et al.. Infect Immun 2001, 69, 7140-7145), have recently been shown to be essential in colonization and infection in an in vivo mouse model. Hence, these two immunogenic proteins could serve as potential vaccine candidates.
- Publication type
- Journal article
- Proteomics, Vol. 6, no. 3 (Feb 2006), pp. 836-844
- Publication year
- FOR Code(s)
- 0601 Biochemistry and Cell Biology
- Immunoblotting; Immunoproteomics; Klebsiella pneumoniae; Outer membrane protein; Vaccine candidate
- WILEY-VCH Verlag
- Publisher URL
- Copyright © 2006 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Peer reviewed