Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis

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Title: Identification of vaccine candidate antigens of an ESBL producing Klebsiella pneumoniae clinical strain by immunoproteome analysis
Author(s): Kurupati, Prathiba; Teh, Boon King; Kumarasinghe, Gamini; Poh, Chit Laa
Abstract: Klebsiella pneumoniae is an opportunistic pathogen which causes pneumoniae, urinary tract infections and septicemia in immunocompromised patients. Hospital outbreaks of multidrug-resistant K. pneumoniae, especially those in neonatal wards, are often caused by strains producing the extended-spectrum-β-lactamases (ESBLs). An immunoproteome based approach was developed to identify candidate antigens of K. pneumoniae for vaccine development. Sera from patients with acute K. pneumoniae infections (n = 55) and a control group of sera from healthy individuals (n = 15) were analyzed for reactivity by Western blot against ESBL K. pneumoniae outer membrane proteins separated by 2-DE. Twenty highly immunogenic protein spots were identified by immunoproteomic analysis. The immunogenic proteins that are most frequently recognized by positive K. pneumoniae sera were OmpA, OmpK36, FepA, OmpK17, OmpW, Colicin I receptor protein and three novel proteins. Two of the vaccine candidate genes, OmpA (Struve et al. Microbiology 2003, 149, 167-176) and FepA (Lai, Y. C. et al.. Infect Immun 2001, 69, 7140-7145), have recently been shown to be essential in colonization and infection in an in vivo mouse model. Hence, these two immunogenic proteins could serve as potential vaccine candidates.
Publication type: Journal article
Source: Proteomics, Vol. 6, no. 3 (Feb 2006), pp. 836-844
Publication year: 2006
FOR Code(s): 0601 Biochemistry and Cell Biology
Keyword(s): Immunoblotting; Immunoproteomics; Klebsiella pneumoniae; Outer membrane protein; Vaccine candidate
Publisher: WILEY-VCH Verlag
ISSN: 1615-9853
Publisher URL: http://dx.doi.org/10.1002/pmic.200500214
Peer reviewed