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Home List of Titles Passive protection against lethal enterovirus 71 infection in newborn mice by neutralizing antibodies elicited by a synthetic peptide
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/81705
- Passive protection against lethal enterovirus 71 infection in newborn mice by neutralizing antibodies elicited by a synthetic peptide
- Foo, Damian Guang Wei; Alonso, Sylvie; Chow, Vincent Tak Kwong; Poh, Chit Laa
- Enterovirus 71 (EV71) infections could lead to high mortalities and neither vaccine nor therapeutic treatment is available. We investigated vaccination with a synthetic peptide SP70 representing a neutralizing linear VP1 epitope of EV71 strain 41 (subgenogroup B4) and passive transfer of anti-SP70 antibodies to protect suckling Balb/c mice against EV71 infectivity. When the mouse anti-SP70 antisera with a neutralizing antibody titer of 1:32 were passively administered to one-day-old suckling mice which had been challenged with a lethal dose of 1000 TCID50 per mouse, the neutralizing anti-SP70 antibodies were able to confer 80% in vivo protection. In contrast, suckling mice which did not receive any anti-SP70 antisera did not survive the viral challenge at day 21 postinfection. Histological examination and real-time RT-PCR assays revealed viral infiltration in small intestines of EV71-infected mice. Interestingly, anti-SP70 antibodies play a major role in the inhibition of EV71 replication in vivo and significantly reduced the viral titer. In conclusion, EV71-neutralizing antibodies elicited by the synthetic peptide SP70 were able to confer good in vivo passive protection against homologous and heterologous EV71 strains in suckling Balb/c mice.
- Publication type
- Journal article
- Microbes and Infection, Vol. 9, no. 11 (Sep 2007), pp. 1299-1306
- Publication year
- FOR Code(s)
- 0605 Microbiology; 1108 Medical Microbiology
- Enterovirus 71; Passive protection; Synthetic peptide; VP1 capsid protein
- Publisher URL
- Copyright © 2007 Elsevier Masson SAS. All rights reserved.
- Peer reviewed