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Home List of Titles Hierarchy of DNA immobilization and hybridization on poly-L-lysine using an atomic force microscopy study
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/1704
- Hierarchy of DNA immobilization and hybridization on poly-L-lysine using an atomic force microscopy study
- Sawant, P.; Watson, Gregory S.; Nicolau, Dan Jr; Myhra, Sverre; Nicolau, Dan V.
- The atomic force microscopy has been used to analyze the immobilization of single stranded DNA on poly-L-lysine-coated glass and subsequent hybridization with complimentary DNA with the Z-threshold parameter and fractal analysis methods. The poly-L-lysine layer, which has a thickness of approximately 7 nm, presents nano-defects that could be critical for DNA immobilization by acting as a nucleation sites for ssDNA and subsequently for dsDNA aggregates. The Z-threshold for the dsDNA aggregates is much larger than for ssDNA, but the statistical fractal dimension is very similar, suggesting a conformal increase of the dimensions of the dsDNA aggregates mainly in the Z-direction, due to an effective ssDNA-ccDNA molecular recognition. This study demonstrates the use of fractal analysis in conjunction with the distribution of heights to evaluate the efficiency of DNA-DNA molecular recognition on surfaces and the impact of nanodefects.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Faculty of Engineering and Industrial Sciences
- Journal of Nanoscience and Nanotechnology, Vol. 5, no. 6 (2005), pp. 951-957
- Publication year
- American Scientific Publishers
- Publisher URL
- Copyright © 2005 American Scientific Publishers. The publisher does not allow institutions to archive either the published version or the accepted manuscript of the paper.
- Peer reviewed