Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/1800

Title

Multi-photon fluorescence microscopy: the response of plant cells to high intensity illumination

Author(s)

Cheng, Ping C.;  Gan, Xiaosong;  Gu, Min;  Huang, Mao K.;  Kao, Fu J.;  Lin, Bai L.;  Wang, Yung S.;  Xu, Ming G.

Abstract

Multi-photon fluorescence microscopy has been cited for its advantage in increased depth penetration due to low linear absorption and scattering coefficient of biological specimen in the near infrared (NIR) range. Because of the need of high peak power for efficiently exciting two-photon fluorescence, the relationship between cell damage and peak power has become an interesting and much debated topic in the applications of multi-photon fluorescence microscopy. It is conceivable that at high illumination intensity, non-linear photochemical processes have impacts on cell physiology and viability in ways much different from low illumination in the linear domain. In this article, we discuss some of the issues in two-photon fluorescence microscopy, including the degree of transparency of the specimen, a comparison of single- and two-photon excited fluorescence spectra, and the cell damage under high intensity illumination, using plant cells as a model.

Publication type

Journal article

Research centre

Swinburne University of Technology. School of Biophysical Science and Electrical Engineering. Centre for Micro-Photonics

Source

Micron, Vol. 32, no. 7 (Oct. 2001), pp. 661-669

Publication year

2001

Keyword(s)

Two-photon fluorescence microscopy;  Cell damage;  Absorption;  Scattering;  Arabidopsis;  Auto-fluorescence;  Plant cell;  Protoplast

Publisher

Pergamon

Format

pp. 661-669

ISSN

0968-4328

Publisher URL

http://dx.doi.org/10.1016/S0968-4328(00)00068-8

Copyright

Copyright © 2001 Elsevier Science Ltd. All rights reserved.

Peer reviewed