Home List of Titles Influence of donor age on development of gonadal tissue from pouch young of the tammar wallaby, Macropus eugenii, after crypreservation and xenografting into mice
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/94570
- Influence of donor age on development of gonadal tissue from pouch young of the tammar wallaby, Macropus eugenii, after crypreservation and xenografting into mice
- Mattiske, D.; Shaw, G.; Shaw, J. M.
- Ovaries from a marsupial, the tammar wallaby (Macropus eugenii), were grafted into a eutherian recipient at known stages of development to ascertain whether normal development would occur. Xenografted ovaries from pouch young < 20 days old, before the onset of meiosis, retained few germ cells and developed tubule-like structures reminiscent of seminiferous cords. Ovaries from 50-day-old pouch young, which contain primordial follicles, developed into antral follicles and corpora lutea within the eutherian host, and produced hormones that stimulated the reproductive tract of the host. The timing of onset of antrum formation and the progress of follicle development were advanced relative to the timing of events in ovaries in situ. Frozen-thawed ovaries from 50-day-old donors developed into preantral follicles, but at a reduced rate and number. This finding shows that gonads of a marsupial species can develop as xenografts in a eutherian, forming large antral follicles. Accelerated follicular development in xenografts provides a potentially valuable model for studying the factors that control follicle development. Assisted reproduction of endangered marsupials may also be feasible using follicles from pouch young grown as xenografts in a eutherian host.
- Publication type
- Journal article
- Reproduction, Vol. 123, no. 1 (Jan 2002), pp. 143-153
- Publication year
- FOR Code(s)
- 0601 Biochemistry and Cell Biology; 1114 Paediatrics and Reproductive Medicine; 1116 Medical Physiology
- Animal tissue; Corpus luteum; Cryopreservation; Endangered species; Frozen section; Genital system; Germ cell; Kangaroos; Macropus eugenii; Marsupials; Meiosis; Ovary development; Seminiferous tubule; Tammar wallabies; Thawing; Xenotransplantation
- Publisher URL
- Copyright © 2002 Society for Reproduction and Fertility.
- Peer reviewed