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Home List of Titles Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/94573
- Graft site and gonadotrophin stimulation influences the number and quality of oocytes from murine ovarian tissue grafts
- Yang, Hsiao Yun; Cox, Shae-Lee; Jenkin, Graham; Findlay, Jock; Trounson, Alan; Shaw, Jillian
- Ovarian tissue cryopreservation and subsequent transplantation can restore fertility in cancer patients. This study used a mouse ovarian grafting model to investigate whether the graft site (bursal cavity, the kidney capsule or subcutaneous) influences the number, fertilization rate and developmental potential of oocytes recovered from grafts and whether using a standard gonadotrophin stimulation protocol would increase oocyte yield from the grafts. Mouse ovarian tissue was grafted into four week old mice and collected three weeks later. Graft recipients were treated either with or without exogenous gonadotrophin stimulation prior to graft collection. Grafted ovaries yielded oocytes that were either at the germinal vesicle (GV) stage or mature metaphase II (MII) stage at collection. These GV oocytes were matured before in vitro fertilization (IVF), while the MII oocytes underwent IVF immediately. Oocytes collected from the oviducts of non-grafted superovulated mice of the same age served as controls. Two-cell embryos were transferred to pseudopregnant recipients and recovered at day 15 of gestation or left to go to term. Graft retrieval and the number of oocytes from each graft were lowest from the subcutaneous graft site. The number of two-cell embryos produced was significantly higher for oocytes from the grafts to the bursa as compared with the other sites. All graft sites gave rise to embryos with comparable implantation rates and developmental potential to fetuses and offspring following transfer. However, the oocytes from grafted ovaries had a significantly lower developmental potential when compared with the control group. Stimulation with exogenous gonadotrophins did not significantly increase oocyte yield from grafted ovaries but did enhance oocyte maturation and development. In conclusion, graft site affects the number and quality of oocytes produced from ovarian grafts.
- Publication type
- Journal article
- Reproduction, Vol. 131, no. 5 (May 2006), pp. 851-859
- Publication year
- FOR Code(s)
- 0601 Biochemistry and Cell Biology; 1114 Paediatrics and Reproductive Medicine; 1116 Medical Physiology
- Mice; Seric gonadotropin
- Publisher URL
- Copyright © 2006 Society for Reproduction and Fertility.
- Peer reviewed