Home List of Titles Mesoporous silica templated biolabels with releasable fluorophores for immunoassays
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150065
- Mesoporous silica templated biolabels with releasable fluorophores for immunoassays
- Cai, Wenyi; Gentle, Ian R.; Lu, Gao Qing; Zhu, Jun-Jie; Yu, Aimin
- A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (~1000-6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was ~50 times higher than the conventional dye labelled antibody system.
- Publication type
- Journal article
- Analytical Chemistry, Vol. 80, no. 14 (Jun 2008), pp. 5401-5406
- Publication year
- FOR Code(s)
- 0301 Analytical Chemistry; 0904 Chemical Engineering
- 3',6'-diacetylfluorescein; Antibodies; Antibody attachments; Antibody labelling; Biolabels; Chemical analysis; Chemical structure; Conventional dyes; Dye molecules; Dyes; Electrolytes; Fluorescein derivative; Fluorescein diacetate; Fluorescence; Immunoassay; Immunology; Layer by layers; Mesoporous silicas; Microscopy electron transmission; Molecular structure; Optimal signals; Organic dyes; Periodic mesoporous organosilica; Porosity; Sandwich immunoassays; Silicon dioxide; Surface property; Temperature; Template methods
- American Chemical Society
- Publisher URL
- Copyright © 2008 American Chemical Society. The American Chemical Society does not allow Swinburne researchers to archive either the accepted manuscript or the published version of the article.
- Peer reviewed