Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150065

Title

Mesoporous silica templated biolabels with releasable fluorophores for immunoassays

Author(s)

Cai, Wenyi;  Gentle, Ian R.;  Lu, Gao Qing;  Zhu, Jun-Jie;  Yu, Aimin

Abstract

A novel class of fluorescent immuno-biolabeling systems with extremely high F/P ratio (~1000-6000) were prepared by combining the template method and layer-by-layer (LbL) technique. Labels were constructed by loading organic dye fluorescein diacetate (FDA) molecules onto hollow periodic mesoporous organosilica (H-PMO) particles followed by ployelectrolyte encapsulation and antibody attachment. The labeling systems were stimuli-responsive to the addition of concentrated NaOH with the loaded dye molecules being released and detected in a well-controlled manner. When applied in sandwich immunoassays, results indicated that the biolabels were immuno-active and generated an optimal signal that was ~50 times higher than the conventional dye labelled antibody system.

Publication type

Journal article

Source

Analytical Chemistry, Vol. 80, no. 14 (Jun 2008), pp. 5401-5406

Publication year

2008

FOR Code(s)

0301 Analytical Chemistry;  0904 Chemical Engineering

Keyword(s)

3',6'-diacetylfluorescein;  Antibodies;  Antibody attachments;  Antibody labelling;  Biolabels;  Chemical analysis;  Chemical structure;  Conventional dyes;  Dye molecules;  Dyes;  Electrolytes;  Fluorescein derivative;  Fluorescein diacetate;  Fluorescence;  Immunoassay;  Immunology;  Layer by layers;  Mesoporous silicas;  Microscopy electron transmission;  Molecular structure;  Optimal signals;  Organic dyes;  Periodic mesoporous organosilica;  Porosity;  Sandwich immunoassays;  Silicon dioxide;  Surface property;  Temperature;  Template methods

Publisher

American Chemical Society

ISSN

0003-2700

Publisher URL

http://dx.doi.org/10.1021/ac800430m

Copyright

Copyright © 2008 American Chemical Society. The American Chemical Society does not allow Swinburne researchers to archive either the accepted manuscript or the published version of the article.

Peer reviewed