Home List of Titles Amperometric nitric oxide biosensor based on the immobilization of hemoglobin on a nanometer-sized gold colloid modified Au electrode
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150035
- Amperometric nitric oxide biosensor based on the immobilization of hemoglobin on a nanometer-sized gold colloid modified Au electrode
- Gu, Hai-Ying; Yu, Ai-Min; Yuan, Su-Su; Chen, Hong-Yuan
- A novel reagentless NO biosensor based on hemoglobin (Hb) immobilized on nanometer-sized gold colloid particles associated with a cysteamine monolayer on gold electrode surface was presented. The experimental optimization was studied in detail. The immobilized Hb displayed an excellent electrocatalytic response to NO. The electrocatalytic currents were proportional to the NO concentration from 1.2 x 10(-8) to 5.0 x 10(-6) M and the detection limit was as low as 4.0 x 10(-9) M (S/N = 3) with good stability. Arginine, ascorbate, nitrite, uric acid, epinephrine and cystine didn't interfere with the determination of NO. The Michaelis-Menten constant (K-m) was determined to be 0.19 mM. This resulting biosensor has been applied to the direct determination of trace NO in raw blood.
- Publication type
- Journal article
- Analytical Letters, Vol. 35, no. 4 (2002), pp. 647-661
- Publication year
- FOR Code(s)
- 0301 Analytical Chemistry
- Adsorption; Gold colloid; Haemoglobin; Horseradish peroxidase; Microsensors; Monolayer films; Nickel; Nitric oxide; Reagentless biosensors; Reduction; Surfaces; Voltammetry
- Marcel Dekker (now an imprint of Taylor & Francis)
- Publisher URL
- Copyright © 2002 by Marcel Dekker, Inc. The copyright policy of the publisher allows the accepted manuscript of the paper to be reproduced here after a publisher-imposed embargo period.
- Peer reviewed