Home List of Titles Separation and determination of di- and tricarboxylic acids in fruits by capillary zone electrophoresis with amperometric detection
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150034
- Separation and determination of di- and tricarboxylic acids in fruits by capillary zone electrophoresis with amperometric detection
- Yang, Wen-Chu; Yu, Ai-Min; Dai, Yi-Qing; Chen, Hong-Yuan
- The method for separation and determination of several di- and tricarboxylic acids, including oxalic, malic, tartaric and citric acids (OX, MA, TA and CI), found in fruit juices by capillary zone electrophoresis with amperometric detection at a copper electrode was developed. Based on cyclic voltammetry (CV) experiments, the detection mechanism for the analytes at a copper electrode was proved to be the formation of complexes between di- and tricarboxylic acids and Cu(II) ion on the electrode surface. Separation was carried out in a pH 6.2 phosphate buffer solution (PBS) containing 0.4mmol/l cetylpridinium bromide (CPB) and 20mmol/l β-cyclodextrin (β-CD) as an electro-osmotic flow (EOF) modifier to reverse the direction of the EOF and a complexing reagent for CPB to improve the separation, respectively. At the working potential of 0.12V (versus SCE), the calibration curves for the analytes were linear with about three orders of magnitude and the detection limits (S/N=3) for them were between 4 and 8fmol. By the method developed here, di- and tricarboxylic acids in four fruit juices, apple, grape, pear and orange juices, were successfully separated and determined.
- Publication type
- Journal article
- Analytica Chimica Acta, Vol. 415, no. 1-2 (Jun 2000), pp. 75-81
- Publication year
- FOR Code(s)
- 0301 Analytical Chemistry
- Amperometric detection; Amperometry; Capillary electrophoresis; Carboxylic acid; Cyclic potentiometry; Food quality; Fruit juice; Tricarboxylic acid
- Publisher URL
- Copyright © 2000 Elsevier Science B.V.
- Peer reviewed