Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150176

Title

Amperometric NADH biosensor based on magnetic chitosan microspheres/poly(thionine) modified glassy carbon electrode

Author(s)

Liu, Ya;  Zhang, Hai-Li;  Lai, Guo-Song;  Yu, Ai-Min;  Huang, Yu-Mei;  Han, De-Yan

Abstract

Novel magnetic chitosan-coated microspheres (MCMSs) were prepared by modifying carbon-coated iron magnetic nanoparticles with chitosan An amperometric dihydronicotinamide adenine dinucleotide (NADH) sensor was constructed based on immobilizing MCMS on the surface of a polythionine (m(-1)) modified glassy carbon elect ode (GCE) The fabrication of MCMS/IPTH film and its electrocatalytic effect on electrochemical oxiclation of NADH were investigated by electrochemical impedance spectroscopy (EIS) and voltammetric methods It was found that the result mg integrated films of PTH and MCMS exhibit high electrocatalytic response to NADH by significantly reduce its overpotential The effects of the experimental variables on the amperometric determination of NADH such as solution pH and working potential wee investigated for optimum analytical performance This electrochemical sensor had a fast response to NADH which was less than 10 s Linear response ranges of 2-10 mu M and 10-100 mu M and a detection limit of 0.51 mu M (S/N = 3) were obtained under the optimum conditions Moreover, the selectivity. stability and reproducibility of this biosensor was evaluated with satisfactory results

Publication type

Journal article

Source

Electroanalysis, Vol. 22, no. 15 (Aug 2010), pp. 1725-1732

Publication year

2010

FOR Code(s)

0301 Analytical Chemistry

Keyword(s)

Biosensors;  Dihydronicotinamide adenine dinucleotide;  Magnetic microspheres;  Polythionine;  Electrocatalysis

Publisher

Wiley

ISSN

1040-0397

Publisher URL

http://dx.doi.org/10.1002/elan.200900544

Copyright

Copyright © 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. The copyright policy of the publisher allows the accepted manuscript of the paper to be reproduced here after a publisher-imposed embargo period.

Peer reviewed