Hydrogen peroxide biosensors based on the immobilization of hemoglobin on amino-silane magnetic nanoparticle-contained carbon paste electrodes

Search Swinburne Research Bank

Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150128

Check Swinburne's subscriptions for full text availability

Title: Hydrogen peroxide biosensors based on the immobilization of hemoglobin on amino-silane magnetic nanoparticle-contained carbon paste electrodes
Author(s): Huang, Yu-Mei; Zhang, Hai-Li; Yu, Ai-Min; Liu, Ya; Wu, Yi-Wei
Abstract: Amino-silane magnetic nanoparticles (A-SMNPs) were mixed with graphite powder to construct A-SMNPs-contained carbon paste electrodes (CPE). Hemoglobin (Hb) was then immobilized on the electrode surface to form Hb/A-SMNPs/CPE biosensors. The biosensor was characterized by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV). The results indicated that Hb was stably immobilized on the electrode and exhibit excellent electrocatalytic activity to the reduction of hydrogen peroxide without the aid of an electron mediator. When used for amperometric measurement of H2O2, the prepared biosensor displayed good sensitivity, selectivity and stability. A linear detection range of H2O2 from 1.0 μmol L-1 to 2.0 mmol L-1 and a detection limit of 0.27 μmol L-1 (S/N = 3) was obtained. The apparent Michaelis-Menten constant was calculated to be 0.85 mmol L-1, indicating that the immobilized Hb had high enzymatic activity to H2O2.
Publication type: Journal article
Source: Sensor Letters, Vol. 8, no. 6 (Dec 2010), pp. 864-870
Publication year: 2010
FOR Code(s): 0301 Analytical Chemistry
Keyword(s): Amino-silane magnetic nanoparticles; Biosensors; Haemoglobin; Hydrogen peroxide; Silica
Publisher: American Scientific Publishers
ISSN: 1546-198X
Publisher URL: http://dx.doi.org/10.1166/sl.2010.1357
Copyright: Copyright © 2010 American Scientific Publishers. All rights reserved. American Scientific Publishers do not allow institutions to archive either the accepted manuscript or the published version of the article.
Peer reviewed