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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150133
- Enzymatically active colloidal crystal arrays
- Yu, Aimin; Liang, Zhijian
- We report the construction of three-dimensional (3D) colloidal crystal arrays (CCA) and hollow colloidal crystal arrays (HCCA) derived from the self-assembly of polyelectrolytes (PE)-coated polystyrene (PS) particles and their use as models of high surface area systems to immobilize peroxidase (POD). POD molecules could infiltrate into the deep layers of CCA and HCCA through their interconnected pores and strongly adsorbed at the PE shell of the colloidal particles. And the total enzyme loading amount and bioactivities increased linearly with the thickness of the CCA till ca. 10 μm. Compared with flat substrates with the same geometrical area, CCA and HCCA exhibit much higher enzyme loading abilities (~43 and 53 times respectively) and the resulting bioactivities (~35 and 41 times respectively) due to their inherently higher surface area and 3D interconnected porous structures. In addition, HCCA could load approximately 30% more POD than CCA because some POD molecules could infiltrate into the interior of the hollow capsule under salt condition.
- Publication type
- Journal article
- Journal of Colloid and Interface Science, Vol. 330, no. 1 (Feb 2009), pp. 144-148
- Publication year
- FOR Code(s)
- 0306 Physical Chemistry (Incl. Structural); 0904 Chemical Engineering; 0914 Resources Engineering and Extractive Metallurgy
- Capsules; Chemical structure; Colloidal crystal arrays; Colloidal crystals; Colloidal particles; Colloids; Crystal structure; Crystallisation; Deep layers; Enzyme activity; Enzyme immobilization; Enzyme loadings; Enzyme substrate; Enzymes; Flat substrates; Geometry; High surface areas; Hollow capsules; Hollow colloidal crystal array; Immobilised enzymes; Interconnected pores; Interconnected porous structures; Interconnection networks; Layer-by-layer; Microarray analysis; Peroxidase; Polyelectrolyte; Polymers; Polystyrenes; Powders; Salt loading; Scanning electron microscopy; Self assembly; Surface areas; Surface properties; Three dimensional imaging
- Academic Press
- Publisher URL
- Copyright © 2008 Elsevier Inc. All rights reserved.
- Peer reviewed