Home List of Titles Biocompatible polypeptide microcapsules via templating mesoporous silica spheres
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150110
- Biocompatible polypeptide microcapsules via templating mesoporous silica spheres
- Yu, Aimin; Gentle, Ian R.; Lu, Gao Qing (Max)
- We reported the stepwise formation of biocompatible poly(l-lysine)/poly(l-glutamic acid) (PLL/PGA) multilayer films on mesoporous silica (MS) spheres via layer-by-layer (LbL) self-assembly technique. In-situ QCM revealed the nonlinear (exponential) growth of PLL/PGA multilayer films at both pH 5.5 and pH 7.0 conditions. ξ-Potential measurements of the multilayer coated particles indicated that the multilayer surface was being charge-overcompensated in each adsorption step, thereby facilitating adsorption of the next oppositely charged polypeptide onto the MS spheres. Hollow polypeptide capsules could be obtained by subsequently removing silica cores in HF solution. By using enzyme-preloaded MS spheres as capsule templates, a general approach was developed for encapsulating enzymes in biocompatible microcapsules with high loading and retained bioactivity. The loading amount for several enzymes with different sizes and their bioactivity after encapsulation were also reported.
- Publication type
- Journal article
- Journal of Colloid and Interface Science, Vol. 333, no. 1 (May 2009), pp. 341-345
- Publication year
- FOR Code(s)
- 0306 Physical Chemistry (Incl. Structural); 0904 Chemical Engineering; 0914 Resources Engineering and Extractive Metallurgy
- Adsorption; Amino acids; Artificial membranes; Biocompatibility; Biocompatible hollow capsules; Biocompatible materials; Catalysts; Charged polypeptides; Crystallisation; Electric potential; Encapsulation; Enzyme activity; Enzymes; Films; HF solutions; High loadings; Hydrofluoric acid; L lysines; Layer-by-layer; L-glutamic acids; Loading amounts; Material coating; Mesoporous silica spheres; Microcapsules; Microspheres; Molecular size; Molecular stability; Multi-layer coated particles; Multilayer films; Multilayer surfaces; Multilayers; Particle size; Peptides; Polyglutamic acid; Polylysine; Polypeptide; Porosity; Quartz crystal microbalance; Self assembly; Silica cores; Silicon dioxide; Surface properties; Synthesis; Templating
- Academic Press
- Publisher URL
- Copyright © 2009 Elsevier Inc. All rights reserved.
- Peer reviewed