Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150110

Title

Biocompatible polypeptide microcapsules via templating mesoporous silica spheres

Author(s)

Yu, Aimin;  Gentle, Ian R.;  Lu, Gao Qing (Max)

Abstract

We reported the stepwise formation of biocompatible poly(l-lysine)/poly(l-glutamic acid) (PLL/PGA) multilayer films on mesoporous silica (MS) spheres via layer-by-layer (LbL) self-assembly technique. In-situ QCM revealed the nonlinear (exponential) growth of PLL/PGA multilayer films at both pH 5.5 and pH 7.0 conditions. ξ-Potential measurements of the multilayer coated particles indicated that the multilayer surface was being charge-overcompensated in each adsorption step, thereby facilitating adsorption of the next oppositely charged polypeptide onto the MS spheres. Hollow polypeptide capsules could be obtained by subsequently removing silica cores in HF solution. By using enzyme-preloaded MS spheres as capsule templates, a general approach was developed for encapsulating enzymes in biocompatible microcapsules with high loading and retained bioactivity. The loading amount for several enzymes with different sizes and their bioactivity after encapsulation were also reported.

Publication type

Journal article

Source

Journal of Colloid and Interface Science, Vol. 333, no. 1 (May 2009), pp. 341-345

Publication year

2009

FOR Code(s)

0306 Physical Chemistry (Incl. Structural);  0904 Chemical Engineering;  0914 Resources Engineering and Extractive Metallurgy

Keyword(s)

Adsorption;  Amino acids;  Artificial membranes;  Biocompatibility;  Biocompatible hollow capsules;  Biocompatible materials;  Catalysts;  Charged polypeptides;  Crystallisation;  Electric potential;  Encapsulation;  Enzyme activity;  Enzymes;  Films;  HF solutions;  High loadings;  Hydrofluoric acid;  L lysines;  Layer-by-layer;  L-glutamic acids;  Loading amounts;  Material coating;  Mesoporous silica spheres;  Microcapsules;  Microspheres;  Molecular size;  Molecular stability;  Multi-layer coated particles;  Multilayer films;  Multilayer surfaces;  Multilayers;  Particle size;  Peptides;  Polyglutamic acid;  Polylysine;  Polypeptide;  Porosity;  Quartz crystal microbalance;  Self assembly;  Silica cores;  Silicon dioxide;  Surface properties;  Synthesis;  Templating

Publisher

Academic Press

ISSN

0021-9797

Publisher URL

http://dx.doi.org/10.1016/j.jcis.2009.01.036

Copyright

Copyright © 2009 Elsevier Inc. All rights reserved.

Peer reviewed