Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150175

Title

The immobilization of hepatocytes on 24 nm-sized gold colloid for enhanced hepatocytes proliferation

Author(s)

Gu, Hai-Ying;  Chen, Zhong;  Sa, Rong-Xiao;  Yuan, Su-Su;  Chen, Hong-Yuan;  Ding, Yi-Tao;  Yu, Ai-Min

Abstract

Bioartificial liver and hepatocyte transplantation is anticipated to supply a temporary metabolic support for candidates of liver transplantation or for patients with fulminant liver failure. An essential restriction of this form is the inability to acquire an enough amount of hepatocytes. Enhancement of the proliferation and differentiated function of hepatocytes is becoming a pursued target. Here, porcine hepatocytes were successfully immobilized on nano-sized gold colloid particles to construct a "hepatocyte/gold colloid" interface at which hepatocytes can be quickly proliferated. The properties of this resulting interface were characterized and confirmed by scanning electron microscopy and atomic force microscopy. The proliferative mechanism of hepatocytes was also discussed. The poliferated hepatocytes could be applied to the clinic based on their excellent functions for the synthesis of protein, glucose and urea as well as lower lactate dehydrogenase release.

Publication type

Journal article

Source

Biomaterials, Vol. 25, no. 17 (Aug 2004), pp. 3445-3451

Publication year

2004

FOR Code(s)

0903 Biomedical Engineering;  0904 Chemical Engineering;  0912 Materials Engineering

Keyword(s)

Animals;  Artificial livers;  Atomic force microscopy;  Biocompatible materials;  Biomaterials;  Biosynthesis;  Cell adhesion;  Cell culture techniques;  Cell division;  Cell proliferation;  Colloidal gold;  Glucose;  Gold colloid;  Hepatocytes;  Immobilised cell;  Liver cell;  Materials testing;  Metabolism;  Miniature swine;  Nanostructured materials;  Nanotechnology;  Nanotubes;  Proliferation;  Proteins;  Scanning electron microscopy;  Suidae;  Surface properties;  Tissue engineering;  Urea

Publisher

Elsevier

ISSN

0142-9612

Publisher URL

http://dx.doi.org/10.1016/j.biomaterials.2003.10.048

Copyright

Copyright © 2003 Elsevier Ltd. All rights reserved.

Peer reviewed