Modeling and optimization of the chiral selectivity of basic analytes in chiral capillary electrophoresis with negatively charged cyclodextrins using electrochemical detection

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Electrochemical detection | Chen, Hong-Yuan

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Title: Modeling and optimization of the chiral selectivity of basic analytes in chiral capillary electrophoresis with negatively charged cyclodextrins using electrochemical detection
Author(s): Yang, Wen-Chu; Yu, Ai-Min; Yu, Xiao-Dong; Chen, Hong-Yuan
Abstract: A mathematical model concerning the separation selectivity of basic analytes in chiral capillary electrophoresis (CE) modified with negatively charged cyclodextrins (CDs) has been presented to describe the dependence of chiral selectivity on the buffer pH and the chiral selector concentration. The electrophoretic method to determine the parameters of the model has also been developed. The model has been tested with racemic epinephrine and isoproterenol as target analytes and sulfonated P-CD as chiral selector. The agreements have been found between the calculated and the measured values. Some significant conclusions to optimize chiral CE separation have been derived from the model and proven by the experiments. Electrochemical detection was used to meet the requirement of the low introduced concentration of analytes.
Publication type: Journal article
Source: Electrophoresis, Vol. 22, no. 10 (Jun 2001), pp. 2025-2031
Publication year: 2001
FOR Code(s): 0301 Analytical Chemistry; 0707 Veterinary Sciences
Keyword(s): Beta-cyclodextrin; Binding; Chiral capillary electrophoresis; Electrochemical detection; Enantiomeric separations; Enantioselective determination; Mathematical models; Neutral cyclodextrins; Selectivity optimisation; Selectors; Sulfated cyclodextrins; Sulfonated beta-cyclodextrin; Theoretical aspects; Tioconazole enantiomers; Zone electrophoresis
Publisher: Wiley
ISSN: 0173-0835
Publisher URL: http://dx.doi.org/10.1002/1522-2683(200106)22:10<2025::AID-ELPS2025>3.0.CO;2-M
Copyright: Copyright © 2001 WILEY-VCH Verlag GmbH, Weinheim, Fed. Rep. of Germany. The copyright policy of the publisher allows the accepted manuscript of the paper to be reproduced here after a publisher-imposed embargo period.
Peer reviewed