Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/150145

Title

Fabrication of thionin-toluene diisocyanate modified electrode and its electrocatalytic oxidation to NADH

Author(s)

Yu, A. M.;  Han, J. L.;  Yang, K. S.;  Zhang, H. L.;  Chen, H. Y.

Abstract

The chemically modified electrode constructed by chemically cross-linking of redox mediator thionin with toluene diisocyanate(TDI) directly at the surface of a spectrographic graphite electrode shows a significant electrocatalytic activity to the oxidation of reduced nicotinamide adenine dinucleotide (NADH) with oxidation overpotential reduced by 310 mV. In the potential range from -0.1 to 0.3 V, the adsorbed thionin-TDI behaves as a one electron and one proton reversible redox process. The modified electrode achieves a steady-state Current of NADH within 20 s and the detection limit is about 1.5 mu M Furthermore, alcohol dehydrogenase (ADH), NAD(+) were cross-linked directly in aid of the reaction of thionin and TDI on the electrode surface to yield an alcohol reagentless sensor without covering a polymer membrane for protection.

Publication type

Journal article

Source

Chemical Research in Chinese Universities, Vol. 12, no. 3 (Aug 1996), pp. 245-252

Publication year

1996

FOR Code(s)

03 Chemical Sciences

Keyword(s)

Adsorption;  Biosensors;  Carbon electrodes;  Catalytic oxidation;  Dihydronicatinamide adenine-dinucleotide;  Electrocatalysis;  Modified electrodes;  Modified graphic electrodes;  NADH;  TN-TDI derivative

Publisher

Jilin Daxue

ISSN

1005-9040

Copyright

Copyright © 1996.

Peer reviewed