Alcohol dehydrogenase (ADH) and the oxidized form of nicotinamide adenine dinucleotide(NAD(+)) were cross-linked directly in aid of the reaction of thionine and toluene diisocyanate (TDI) on the surface of glassy carbon electrode to yield a reagentless alcohol sensor. The NAD(+) was converted to NADH by oxidation of alcohol catalyzed by ADH and the NADH formed was oxidized electrochemically to the original NAD(+) by thionine-TDI mediator, thus giving the catalytic current for target analysis. The peak current was linearly related to the concentration of alcohol in the range of 1-15 mmol/L. The influence of various experimental conditions was also explored for optimum analytical performance. The sensor remains relatively stable for about 15 days.
Chemical Journal of Chinese Universities (Gaodeng Xuexiao Huaxue Xuebao),
Vol. 16, no. 8 (1995), pp. 1204-1206