The electrochemistry of cytochrome C was investigated as a spectrographic graphite electrode. In phosphate buffer solution (pH = 7.0), cytochrome C showed stable and quasi-reversible response. The formal potential E0' was 0.015 V (at 25-degrees-C, vs. SCE) and the heterogeneous electron transfer rate constant k(s) obtained varied form 1.10 X 10(-3) cm . s-1 to 1.80 X 10(-3) cm . s-1. The thermodynamic parameters of the electron transfer reaction of cytochrome C was also estimated. Furthermore, the effect of the various electrode surface states on the electrochemistry of cytochrome C was discussed.
Chemical Research in Chinese Universities,
Vol. 11, no. 1 (1995), pp. 22-27