Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/2044

Title

Pseudomonas extremorientalis sp. nov., isolated from a drinking water resevoir

Author(s)

Christen, Richard;  Gorshkova, Nataliya M.;  Hayashi, Karin;  Ivanova, Elena P.;  Kalinovskaya, Nataliya I.;  Kuznetsova, Tatyana A.;  Lysenko, Anatoly M.;  Mikhailov, Valery V.;  Nicolau, Dan V.;  Sawabe, Tomoo;  Zhukova, Natalia V.

Abstract

On the basis of phenotypic and genotypic characteristics and 16S rDNA sequence analysis, a novel species belonging to the genus Pseudomonas sensu stricto was identified. The saprophytic, fluorescent bacterium, designated KMM 3447(T), was isolated from a drinking water reservoir near Vladivostok City, Russia. The novel organism was a Gram-negative, aerobic, rod-shaped bacterium that produced a cyclic depsipeptide with surface-active properties. It degraded casein, but did not degrade gelatin, starch, agar or Tween 80. The bacterium was also haemolytic. Growth of the novel bacterium occurred between 4 and 35 degrees C. The predominant cellular fatty acids of the novel pseudomonad were C(16:0), C(16:1(n-7)), C(18:1(n-7)) and C(17:0 cyclo); branched fatty acids were only found in trace amounts. The G+C content of the novel bacterium was 61.0 mol%. 16S rDNA sequence analysis indicated that the novel bacterium had a clear affiliation with Pseudomonas fluorescens and species closely related to this recognized pseudomonad. DNA--DNA hybridization experiments showed that the novel bacterium bound at low levels (27--53%) with the DNA of the type strains of its nearest phylogenetic relatives, namely Pseudomonas tolaasii, Pseudomonas veronii, Pseudomonas orientalis and Pseudomonas rhodesiae, indicating that the novel bacterium represented a novel species within the genus Pseudomonas, for which the name Pseudomonas extremorientalis is proposed; the type strain is KMM 3447(T) (=LMG 19695(T)).

Abstract

2003

Publication type

Journal article

Research centre

Swinburne University of Technology. Industrial Research Institute Swinburne

Source

International Journal of Systematic and Evolutionary Microbiology, Vol. 52, no. 6 (2002), pp. 2113-2120

Publication year

2002

Publisher

Society for General Microbiology

Format

pp. 2113-2120

ISSN

1466-5026

Publisher URL

International Journal of Systematic and Evolutionary Microbiology

Publisher URL

http://ijs.sgmjournals.org/cgi/content/abstract/52/6/2113

Copyright

Copyright © 2002 by Society for General Microbiology

Peer reviewed