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Title

Multiphoton fluorescence microscopic imaging through double-layer turbid tissue media

Author(s)

Deng, Xiaoyuan;  Gan, Xiaosong;  Gu, Min

Abstract

Image formation in multiphoton fluorescence microscopy through double-layer turbid tissue media is investigated using Monte Carlo simulation. With the help of the concept of the effective point spread function, the relationship of image resolution and signal level to the thickness and scattering properties of the double-layer turbid media under single-, two-, and three-photon excitation is revealed. Results show that for a double-layer turbid medium of a given thickness, small particles in the top layer result in a quicker degradation of signal level than large particles in the top layer. This model is then applied to study the penetration depth of multiphoton fluorescence microscopy through human skin tissue which exhibits a layered structure. It is predicated that using 3p excitation leads to a signal level up to two orders of magnitude higher than that under 2p excitation, while diffraction-limited image resolution can be maintained for skin tissue of thickness up to 500 µm.

Publication type

Journal article

Research centre

Swinburne University of Technology. School of Biophysical Science and Electrical Engineering

Source

Journal of Applied Physics, Vol. 91, no. 7 (2002), 4659-4665

Publication year

2002

Keyword(s)

Bio-optics;  Biological techniques;  Biological tissues;  Fluorescence spectroscopy;  Image resolution;  Monte Carlo methods;  Multiphoton spectroscopy;  Optical microscopy;  Skin;  Turbidity;  Two-photon spectroscopy

Publisher

American Institute of Physics

ISSN

0021-8979

Publisher URL

http://dx.doi.org/10.1063/1.1459107

Copyright

Copyright © 2002 American Institute of Physics. Paper reproduced here in accordance with the copyright policy of the publisher.

Full text

Peer reviewed