Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/2088

Title

Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549T

Author(s)

Alekseeva, Yulia V.;  Alexeeva, Yulia V.;  Bakunina, Irina Y.;  Ivanova, Elena P.;  Mikhailov, Valery V.;  Zvaygintseva, Tatiyana N.

Abstract

Aims: The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549T. Methods and Results: Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging from 2·5 g l-1 to 10 g l-1, while the presence of both yeast extract and glucose did not affect enzyme production. The activity of fucoidan hydrolase and laminaranase increased up to 4·83 µM h-1 mg-1 and 19·23 µM h-1 mg-1 protein, respectively, in growth media containing xylose (1·0 g l-1), laminarin (0·5 g l-1) or alginate (0·5 g l-1), and production of b-N-acetylglucosaminidase substantially increased in the presence of fucoidan (0·5 g l-1) or galactose (1 g l-1). All polysaccharides tested in concentrations of 0·5 g l-1 fucoidan and 0·2 g l-1 fucose induced production of alginase (up to 5·06 µM h-1 mg-1 protein). Conclusions: The production of glycosidases is not only stimulated by the presence of algal polysaccharides, but may also be stimulated by monosaccharides (e.g. xylose). Significance and Impact of the Study: The production of glycosidases by Pseudoalteromonas issachenkonii KMM 3549T was significantly improved by using a simple nutrient medium containing peptone (2·5 g l-1) and xylose (5·0 g l-1) in 100% natural seawater.

Publication type

Journal article

Research centre

Swinburne University of Technology. Industrial Research Institute Swinburne

Source

Letters in applied microbiology, Vol. 35, no. 4 (Oct. 2002), pp. 343-346

Publication year

2002

Publisher

Blackwell Science

Format

pp. 343-346

ISSN

0266-8254

Publisher URL

http://dx.doi.org/doi:10.1046/j.1472-765X.2002.01189.x

Copyright

© 2002 The Society for Applied Microbiology.

Peer reviewed