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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/2088
- Optimization of glycosidases production by Pseudoalteromonas issachenkonii KMM 3549T
- Alekseeva, Yulia V.; Alexeeva, Yulia V.; Bakunina, Irina Y.; Ivanova, Elena P.; Mikhailov, Valery V.; Zvaygintseva, Tatiyana N.
- Aims: The present work aimed to design an optimized medium to yield a higher production of glycosides by Pseudoalteromonas issachenkonii KMM 3549T. Methods and Results: Higher levels of fucoidan hydrolase, alginase, laminaranase and b-N-acetylglucosaminidase production were obtained with peptone concentrations ranging from 2·5 g l-1 to 10 g l-1, while the presence of both yeast extract and glucose did not affect enzyme production. The activity of fucoidan hydrolase and laminaranase increased up to 4·83 µM h-1 mg-1 and 19·23 µM h-1 mg-1 protein, respectively, in growth media containing xylose (1·0 g l-1), laminarin (0·5 g l-1) or alginate (0·5 g l-1), and production of b-N-acetylglucosaminidase substantially increased in the presence of fucoidan (0·5 g l-1) or galactose (1 g l-1). All polysaccharides tested in concentrations of 0·5 g l-1 fucoidan and 0·2 g l-1 fucose induced production of alginase (up to 5·06 µM h-1 mg-1 protein). Conclusions: The production of glycosidases is not only stimulated by the presence of algal polysaccharides, but may also be stimulated by monosaccharides (e.g. xylose). Significance and Impact of the Study: The production of glycosidases by Pseudoalteromonas issachenkonii KMM 3549T was significantly improved by using a simple nutrient medium containing peptone (2·5 g l-1) and xylose (5·0 g l-1) in 100% natural seawater.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Industrial Research Institute Swinburne
- Letters in applied microbiology, Vol. 35, no. 4 (Oct. 2002), pp. 343-346
- Publication year
- Blackwell Science
- pp. 343-346
- Publisher URL
- © 2002 The Society for Applied Microbiology.