Feasibility of using carboxylic-rich polymeric surfaces for the covalent binding of oligonucleotides for microPCR applications

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Title: Feasibility of using carboxylic-rich polymeric surfaces for the covalent binding of oligonucleotides for microPCR applications
Author(s): Grodzinski, Piotr; Ivanova, Elena P.; Nicolau, Dan V.; Oliveira, Anna; Papiernik, Michal; Sbarski, Igor; Smekal, Thomas
Abstract: The chemical binding of oligonucleotide/DNA on polystyrene-related polymeric surfaces has been investigated using contact angle measurements, x-ray photoelectron spectroscopy and gravimetric analysis. The results of the covalent attachment of the phosphorylated oligonucleotides using the hetero-bifunctional cross linker 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride to polystyrene-co-maleic anhydride (PSMAA) and polystyrene-co-maleic acid (PSMA) are described. The immobilization efficiency of covalently coupled 26-mer oligonucleotides to polymeric surfaces was estimated as 0.3 × 1010 and 0.1 × 109 molecules mm-2 for PSMA and PSMAA, respectively. The results suggest that, although the covalent binding on PSMAA per se is not capable of the high density of DNA required by micro-PCR applications, the method has the potential to be used as a cheap alternative for other low-cost, less DNA-sensitive applications such as disposable biosensors.
Publication type: Journal article
Research centre: Swinburne University of Technology. Industrial Research Institute Swinburne
Source: Smart materials and structures, Vol. 11, no. 5 (2002), pp. 783-791
Publication year: 2002
Publisher: Institute of Physics Publishing
Format: pp. 783-791
ISSN: 0964-1726
Publisher URL: http://dx.doi.org/doi:10.1088/0964-1726/11/5/325
Peer reviewed