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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/192853
- Electrophoretic chip for high-fidelity fractionation of double-stranded DNA
- Sun, Kai; Li, Zheyu; Ueno, Kosei; Juodkazis, Saulius; Noji, Sumihare; Misawa, Hiroaki
- We report the high fidelity, on-chip fractionation of selected segments front an electrophoretic flow of separated fragments. dsDNA fragments (10-330 base pairs (bp)) were initially separated using a 6.5 cm long channel with an electric field strength of 150 V/cm. As an example of the fractionation process, a target fragment of 20 bp was selected and extracted from the separation channel. The extraction was confirmed and evaluated by fluorescence imaging. High resolution and extraction fidelity were achieved by introducing new procedures for (i) extraction channel-blocking and (ii) segment transfer with cleaning. These procedures are necessary for the development of a practical, fully automated multitarget fractionation electrophoretic chip. A kind of CCD image processing method was introduced to monitor, control, and evaluate the procedure of fractionation. The resolution limits of the separation and extraction are discussed.
- Publication type
- Journal article
- Electrophoresis, Vol. 28, no. 10 (May 2007), pp. 1572-1578
- Publication year
- FOR Code(s)
- 0301 Analytical Chemistry; 0707 Veterinary Sciences
- CCD; Double-stranded DNA; Electrophoretic chip; Extraction; Fractionation
- Wiley-VCH Verlag
- Publisher URL
- Copyright © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.
- Additional information
- The authors acknowledge support from a Grant-in-Aid from the Ministry of Education, Science, Sports, and Culture, Japan (No. 17360110).
- Peer reviewed