Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/192853

Title

Electrophoretic chip for high-fidelity fractionation of double-stranded DNA

Author(s)

Sun, Kai;  Li, Zheyu;  Ueno, Kosei;  Juodkazis, Saulius;  Noji, Sumihare;  Misawa, Hiroaki

Abstract

We report the high fidelity, on-chip fractionation of selected segments front an electrophoretic flow of separated fragments. dsDNA fragments (10-330 base pairs (bp)) were initially separated using a 6.5 cm long channel with an electric field strength of 150 V/cm. As an example of the fractionation process, a target fragment of 20 bp was selected and extracted from the separation channel. The extraction was confirmed and evaluated by fluorescence imaging. High resolution and extraction fidelity were achieved by introducing new procedures for (i) extraction channel-blocking and (ii) segment transfer with cleaning. These procedures are necessary for the development of a practical, fully automated multitarget fractionation electrophoretic chip. A kind of CCD image processing method was introduced to monitor, control, and evaluate the procedure of fractionation. The resolution limits of the separation and extraction are discussed.

Publication type

Journal article

Source

Electrophoresis, Vol. 28, no. 10 (May 2007), pp. 1572-1578

Publication year

2007

FOR Code(s)

0301 Analytical Chemistry;  0707 Veterinary Sciences

Keyword(s)

CCD;  Double-stranded DNA;  Electrophoretic chip;  Extraction;  Fractionation

Publisher

Wiley-VCH Verlag

ISSN

0173-0835

Publisher URL

http://dx.doi.org/10.1002/elps.200600685

Copyright

Copyright © 2007 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.

Additional information

The authors acknowledge support from a Grant-in-Aid from the Ministry of Education, Science, Sports, and Culture, Japan (No. 17360110).

Peer reviewed