Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/198472

Title

Preventing protein adsorption from a range of surfaces using an aqueous fish protein extract

Author(s)

Pillai, Saju;  Arpanaei, Ayyoob;  Meyer, Rikke L.;  Birkedal, Victoria;  Gram, Lone;  Besenbacher, Flemming;  Kingshott, Peter

Abstract

We utilize an aqueous extract of fish proteins (FPs) as a coating for minimizing the adsorption of fibrinogen (Fg) and human serum albumin (HSA). The surfaces include stainless steel (SS), gold (Au), silicon dioxide (SiO 2), and poly(styrene) (PS). The adsorption processes (kinetics and adsorbed mass) are followed by quartz crystal microbalance with dissipation (QCM-D). Complementary surface information is provided by X-ray photoelectron spectroscopy (XPS) and atomic force microscopy (AFM). QCM-D shows no mass increases to any of the FPcoated surfaces upon treating with Fg or HSA. Also, when Fg- or HSA-coated surfaces are exposed to the FPs, a significant increase in adsorbed mass occurs because the FPs are highly surface-active displacing Fg. Additionally, fluorescence microscopy confirms that very little Fg adsorbs to the FP-coated surfaces. We propose that FP coatings prevent protein adsorption by steric stabilization and could be an alternative method for preventing unwanted bioadhesion on medical materials.

Publication type

Journal article

Source

Biomacromolecules, Vol. 10, no. 10 (Oct 2009), pp. 2759-2766

Publication year

2009

FOR Code(s)

03 Chemical Sciences;  06 Biological Sciences;  09 Engineering

Keyword(s)

Adsorption;  Adsorption process;  Alternative methods;  Animals;  Aqueous extracts;  Aqueous solution;  Atlantic cod;  Atomic force microscopy;  Atomic spectroscopy;  Bio-adhesion;  Coated surface;  Coatings;  Fibrinogen;  Fish protein;  Fishes;  Fluorescence microscopy;  Gold;  Human;  Human serum albumins;  Kinetics;  Mass increase;  Medical materials;  Nonhuman;  Oxide minerals;  Polystyrene;  Protein adsorption;  Protein extract;  Proteins;  Quartz;  Quartz crystal microbalance with dissipation;  Quartz crystal microbalances;  Silica;  Silicon compounds;  Silicon dioxide;  Silicon oxides;  Solvent extraction;  Stainless steel;  Stereospecificity;  Steric stabilisation;  Styrene;  Surface information;  Surface properties;  Surfaces;  X ray photoelectron spectroscopy

Publisher

American Chemical Society

ISSN

1525-7797

Publisher URL

http://dx.doi.org/10.1021/bm900589r

Copyright

Copyright © 2009 American Chemical Society.

Peer reviewed