Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/198636

Title

Fluorescent aromatic platforms for cell patterning

Author(s)

De La Fuente, Jesus M.;  Andar, Abhay;  Gadegaard, Nikolaj;  Berry, Catherine C.;  Kingshott, Peter;  Riehle, Mathis O.

Abstract

This paper describes a simple experimental method of patterning fluorescent organic dyes, fluorescein, and rhodamine on gold substrates by microcontact printing techniques. The development of this step-by-step protocol has allowed us to prepare striped and squared micropatterns with poly(ethylene glycol) (PEG) linkers terminated by these fluorophores using a fast, easy, and inexpensive technique. Although the rest of the surface was covered with aliphatic molecules (methyl terminated), human fibroblasts demonstrated an unexpected response, aligning themselves according to the aromatic patterns, despite the presence of PEG, which is a cell resistant molecule, in the fluorescent regions.

Publication type

Journal article

Source

Langmuir, Vol. 22, no. 13 (Jun 2006), pp. 5528-5532

Publication year

2006

FOR Code(s)

0306 Physical Chemistry (Incl. Structural);  0903 Biomedical Engineering;  0904 Chemical Engineering

Keyword(s)

Aromatic compounds;  Biocompatible coated materials;  Biomaterial;  Cell adhesion;  Cell line;  Cells;  Chemical structure;  Chemistry;  Dyes;  Fibroblasts;  Fluorescein;  Fluorescence;  Fluorescent dyes;  Fluorophores;  Gold;  Materials testing;  Molecular structure;  Organic dyes;  Physiology;  Polyethylene glycols;  Scanning electron microscopy;  Substrates;  Synthesis

Publisher

American Chemical Society

ISSN

0743-7463

Publisher URL

http://dx.doi.org/10.1021/la053045s

Copyright

Copyright © 2006 American Chemical Society.

Peer reviewed