Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/198649

Title

Two-dimensional patterning of thin coatings for the control of tissue outgrowth

Author(s)

Thissen, Helmut;  Johnson, Graham;  Hartley, Patrick G.;  Kingshott, Peter;  Griesser, Hans J.

Abstract

Control of the precise location and extent of cellular attachment and proliferation, and of tissue outgrowth is important in a number of biomedical applications, including biomaterials and tissue engineered medical devices. Here we describe a method to control and direct the location and define boundaries of tissue growth on surfaces in two dimensions. The method relies on the generation of a spatially defined surface chemistry comprising protein adsorbing and non-adsorbing areas that allow control over the adsorption of cell-adhesive glycoproteins. Surface modification was carried out by deposition of thin acetaldehyde and allylamine plasma polymer coatings on silicon wafer and FEP substrates, followed by grafting of a protein resistant layer of poly(ethylene oxide). Spatially controlled patterning of the surface chemistry was achieved by masking during plasma polymerization. XPS and AFM were used to provide evidence of successful surface modifications. Adsorption of the extracellular matrix protein collagen I followed by tissue outgrowth experiments with bovine corneal epithelial tissue for up to 21 days showed that two-dimensional control over tissue outgrowth is achievable with our patterning method over extended time frames. The method promises to be an effective tool for use in a number of in vitro and in vivo applications.

Publication type

Journal article

Source

Biomaterials, Vol. 27, no. 1 (Jan 2006), pp. 35-43

Publication year

2006

FOR Code(s)

0903 Biomedical Engineering;  0904 Chemical Engineering;  0912 Materials Engineering

Keyword(s)

Acetaldehyde;  Adsorption;  Allylamine;  Animals;  Atomic force microscopy;  Biocompatible materials;  Biomedical engineering;  Bovinae;  Cattle;  Cell adhesion;  Cell culture techniques;  Coatings;  Collagen;  Collagen type I;  Cornea;  Electron probe microanalysis;  Epithelial cells;  Extracellular matrix;  Glycoproteins;  Growth kinetics;  In vitro test;  Plasma polymerisation;  Polyethylene glycols;  Polyethylene oxides;  Polymerisation;  Polymers;  Proteins;  Regeneration;  Silicon;  Silicon wafers;  Surface analysis;  Surface chemistry;  Surface modification;  Surface properties;  Surface treatment;  Thin coatings;  Tissue;  Tissue engineering;  Tissue growth;  Tissue outgrowth;  Unclassified drug;  X-ray photoelectron spectroscopy

Publisher

Elsevier

ISSN

0142-9612

Publisher URL

http://dx.doi.org/10.1016/j.biomaterials.2005.05.037

Copyright

Copyright © 2005 Elsevier Ltd. All rights reserved.

Peer reviewed