Production of active lysozyme films by matrix assisted pulsed laser evaporation at 355 nm

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/198601

Title: Production of active lysozyme films by matrix assisted pulsed laser evaporation at 355 nm
Author(s): Purice, Andreea; Schou, Jørgen; Kingshott, Peter; Dinescu, Maria
Abstract: Thin lysozyme films have been produced in a dry environment by MAPLE (matrix assisted pulsed laser evaporation) from a water ice matrix irradiated by laser light at 355 nm above the absorption threshold of the protein. A significant part of the lysozyme molecules are transferred to the film without decomposition and the protein activity is preserved. The film deposition rate for 1 wt% lysozyme shows a clear maximum of about 1 ng/cm2 per shot for a moderate fluence of 2 J/cm2, which is about one-half of the deposition rate from a pressed (100%) lysozyme target.
Publication type: Journal article
Source: Chemical Physics Letters, Vol. 435, no. 4-6 (Feb 2007), pp. 350-353
Publication year: 2007
FOR Code(s): 0202 Atomic, Molecular, Nuclear, Particle and Plasma Physics; 0306 Physical Chemistry (Incl. Structural); 1007 Nanotechnology
Keyword(s): Absorption; Absorption threshold; Biofilms; Decomposition; Deposition; Evaporation; Lysozyme films; MAPLE; Matrix assisted pulsed laser evaporation; Pulsed laser applications; Water ice matrix
Publisher: Elsevier
ISSN: 0009-2614
Publisher URL: http://dx.doi.org/10.1016/j.cplett.2006.12.078
Peer reviewed