Home List of Titles Growth of thin films of low molecular weight proteins by matrix assisted pulsed laser evaporation (MAPLE)
Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/201866
- Growth of thin films of low molecular weight proteins by matrix assisted pulsed laser evaporation (MAPLE)
- Matei, A.; Schou, J.; Constantinescu, C.; Kingshott, P.; Dinescu, M.
- Thin films of lysozyme and myoglobin grown by matrix assisted pulsed laser evaporation (MAPLE) from a water ice matrix have been investigated. The deposition rate of these two low molecular weight proteins (lysozyme: 14307 amu and myoglobin: 17083 amu) exhibits a maximum of about 1–2 ng/cm^2 per pulse at a fluence of 1–2 J/cm^2 and decreases slowly with increasing fluence. This rate is presumably determined by the matrix rather by the proteins. A significant fraction of the proteins are intact in the film as determined by MALDI (Matrix assisted laser desorption ionization) spectrometry. The results for lysozyme demonstrate that the fragmentation rate of the proteins during the MAPLE process is not influenced by the pH of the water solution prior to freezing.
- Publication type
- Journal article
- Research centre
- Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Industrial Research Institute Swinburne
- Applied Physics A: Materials Science and Processing, Vol. 105, no. 3 (Nov 2011), pp. 629-633
- Publication year
- FOR Code(s)
- 0204 Condensed Matter Physics
- Growth; MAPLE; Matrix assisted pulsed laser evaporation; Molecular weight proteins; Thin films
- Publisher URL
- Copyright © Springer-Verlag 2011.
- Peer reviewed