AFM analysis of the formation of DNA aggregates on polymeric biochips

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/2406

Title: AFM analysis of the formation of DNA aggregates on polymeric biochips
Author(s): Myhra, Sverre; Nicolau, Dan V.; Sawant, Prashant; Watson, Gregory S.
Abstract: The demand for polymer-based DNA microarrays will increase because of their cost-effectiveness, biocompatibility and easy processing. However not all polymers are ideal substrates because of different chemical interactions of polymeric substrates with the DNA molecules. Results from AFM analysis of DNA immobilised on polymeric surfaces are evaluated using fractality, Gaussian distribution and lateral force imaging. It has been found that the nanosize defects in the substrate, such as poly-l-lysine, plays an important role in the growth of DNA aggregates in a vertical direction, whereas the covalent binding of DNA molecules on NHS-functionalised cyclo-olefin copolymer leads to the lateral growth of DNA aggregates.
Publication type: Conference paper
Research centre: Swinburne University of Technology. Faculty of Engineering and Industrial Sciences
Source: Proceedings of Biomedical Applications of Micro- and Nanoengineering 2, 13-15 December 2005, Sydney, New South Wales, Vol. 5651, pp. 153-159
Publication year: 2005
Publisher: SPIE
Format: Vol. 5651, pp. 153-159
ISBN: 081945611X
Publisher URL: http://dx.doi.org/10.1117/12.584142
Peer reviewed