Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/2422

Title

Development of an electro-responsive platform for the controlled transfection of mammalian cells

Author(s)

Hayes, J. P.;  Hook, A. L.;  Thissen, H.;  Voelcker, N.

Abstract

The recent development of living microarrays as novel tools for the analysis of gene expression in an in-situ environment promises to unravel gene function within living organisms. In order to significantly enhance microarray performance, we are working towards electro-responsive DNA transfection chips. This study focuses on the control of DNA adsorption and desorption by appropriate surface modification of highly doped p++ silicon. Silicon was modified by plasma polymerisation of allylamine (ALAPP), a non-toxic surface that sustains cell growth. Subsequent high surface density grafting of poly(ethylene oxide) formed a layer resistant to biomolecule adsorption and cell attachment. Spatially controlled excimer laser ablation of the surface produced micron resolution patterns of re-exposed plasma polymer whilst the rest of the surface remained non-fouling. We observed electro-stimulated preferential adsorption of DNA to the ALAPP surface and subsequent desorption by the application of a negative bias. Cell culture experiments with HEK 293 cells demonstrated efficient and controlled transfection of cells using the expression of green fluorescent protein as a reporter. Thus, these chemically patterned surfaces are promising platforms for use as living microarrays.

Publication type

Conference paper

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences

Source

Proceedings of the SPIE Biomedical Applications of Micro- and Nanoengineering II Conference, Sydney, New South Wales, Australia, 13-15 December 2004, Vol. 5651, pp. 418-425

Publication year

2005

Publisher

Springer-Verlag

ISBN

081945611X

Publisher URL

http://dx.doi.org/10.1117/12.606789

Copyright

Copyright © 2005 SPIE -- The International Society for Optical Engineering.

Peer reviewed