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Title

T cell protein tyrosine phosphatase attenuates T cell signaling to maintain tolerance in mice

Author(s)

Wiede, Florian;  Shields, Benjamin J.;  Chew, Sock Hui;  Kyparissoudis, Konstantinos;  van Vliet, Catherine;  Galic, Sandra;  Tremblay, Michel L.;  Russell, Sarah M.;  Godfrey, Dale I.;  Tiganis, Tony

Abstract

Many autoimmune diseases exhibit familial aggregation, indicating that they have genetic determinants. Single nucleotide polymorphisms in PTPN2, which encodes T cell protein tyrosine phosphatase (TCPTP), have been linked with the development of several autoimmune diseases, including type 1 diabetes and Crohn's disease. In this study, we have identified TCPTP as a key negative regulator of TCR signaling, which might explain the association of PTPN2 SNPs with autoimmune disease. We found that TCPTP dephosphorylates and inactivates Src family kinases to regulate T cell responses. Using T cell-specific TCPTP-deficient mice, we established that TCPTP attenuates T cell activation and proliferation in vitro and blunts antigen-induced responses in vivo. TCPTP deficiency lowered the in vivo threshold for TCR-dependent CD8(+) T cell proliferation. Consistent with this, T cell-specific TCPTP-deficient mice developed widespread inflammation and autoimmunity that was transferable to wild-type recipient mice by CD8(+) T cells alone. This autoimmunity was associated with increased serum levels of proinflammatory cytokines and anti-nuclear antibodies, T cell infiltrates in non-lymphoid tissues, and liver disease. These data indicate that TCPTP is a critical negative regulator of TCR signaling that sets the threshold for TCR-induced naive T cell responses to prevent autoimmune and inflammatory disorders arising.

Publication type

Journal article

Research centre

Swinburne University of Technology. Faculty of Engineering and Industrial Sciences. Centre for Micro-Photonics

Source

Journal of Clinical Investigation, Vol. 121, no. 12 (Dec 2011), pp. 4758-4774

Publication year

2011

FOR Code(s)

11 Medical and Health Sciences

Keyword(s)

Autoimmune diseases;  Protein tyrosine phosphatases;  T cell protein tyrosine phosphatase;  T cells;  TCPTP

Publisher

American Society for Clinical Investigation

ISSN

0021-9738

Publisher URL

http://dx.doi.org/10.1172/jci59492

Copyright

Copyright © 2011 The American Society for Clinical Investigation. The published version is reproduced in accordance with the copyright policy of the publisher.

Additional information

The authors acknowledge support from the NHMRC of Australia.

Full text

Peer reviewed