Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/214361

Title

Minimization of protein adsorption on poly(vinylidene fluoride)

Author(s)

Ademovic, Z.;  Klee, D.;  Kingshott, P.;  Kaufmann, R.;  Hocker, H.

Abstract

Surfaces covered with polyethylene glycol (PEG) have been shown to be biocompatible because PEG yields nonimmunogenicity, nonantigenicity and protein rejection. To produce a biocompatible surface coating, we have developed a method for grafting PEG onto modified poly(vinylidene fluoride) (PVDF) films. The first step was to create carboxy groups on the PVDF surface following covalente coupling of polyethylenimine (PEI) to achieve high density of amino groups. These surface amines were reacted with formyl-terminated PEG's with various molecular weight. The modified PVDF surface was characterized by means of static contact angle measurements, infrared (IR) spectroscopy and X-ray photoelectron spectroscopy (XPS). The influence of the chain length on lysozyme repellence was investigated by means of surface-MALDI-Tof mass spectrometry (Surface-MALDI-Tof-MS). Lysozyme adsorption was significantly suppressed on the PEG 5000 modified PVDF surface.

Publication type

Journal article

Source

Biomolecular Engineering, Vol. 19, no. 2-6 (Aug 2002), pp. 177-182

Publication year

2002

FOR Code(s)

06 Biological Sciences;  10 Technology

Keyword(s)

Adsorption;  Biocompatibility;  Biocompatible coated materials;  Biomaterial;  Chemistry;  Comparative study;  Contact angle;  Controlled study;  Lysozyme;  Macrogol derivative;  Mass spectrometry;  Materials testing;  Matrix assisted laser desorption ionization time of flight mass spectrometry;  Muramidase;  Nonhuman;  PEG;  Polyethylene glycols;  Polyvinyl derivative;  Polyvinylidene fluoride;  Polyvinyls;  Protein;  Protein adsorption;  Proteins;  PVDF;  Sensitivity and specificity;  Surface property;  Surface-MALDI-Tof-MS;  Synthesis;  XPS;  X ray photoelectron spectroscopy

Publisher

Elsevier

ISSN

1389-0344

Publisher URL

http://dx.doi.org/10.1016/s1389-0344(02)00020-5

Copyright

Copyright © 2002 Elsevier Science B.V.

Peer reviewed