Ultrathin functional star PEG coatings for DNA microarrays

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Title: Ultrathin functional star PEG coatings for DNA microarrays
Author(s): Ameringer, Thomas; Hinz, Michael; Mourran, Claudia; Seliger, Hartmut; Groll, Juergen; Moeller, Martin
Abstract: In this study, star PEG coatings on glass substrates have been used as support material for oligonucleotide microarrays. These coatings are prepared from solutions of six armed star shaped prepolymers that carry reactive isocyanate endgroups. As described earlier, such films prevent the adsorption of proteins and the adhesion of cells but can easily be functionalized for specific biological recognition. Here we used the high functionality of these coatings for the covalent immobilization of amino terminated 20mer oligonucleotides, both by microcontact printing and spotting techniques. The permanent immobilization of fluorescently labeled DNA as well as hybridization of 20mer oligonucleotides have been monitored by fluorescence microscopy. The hybridization efficiency as determined by fluorescence intensity varied from 30% to 80% depending on the way of layer preparation. The direct spotting without additional activation and blocking steps of the surface demonstrates the potential of star PEG coatings as ultrathin surface modification for microarrays.
Publication type: Journal article
Source: Biomacromolecules, Vol. 6, no. 4 (May 2005), pp. 1819-1823
Publication year: 2005
FOR Code(s): 03 Chemical Sciences; 06 Biological Sciences; 09 Engineering
Keyword(s): Adsorption; Amino terminal sequence; Cell adhesion; Cell immobilisation; Chemical modification; Coatings; Deoxyribonucleic acid; DNA microarrays; Fluorescence microscopy; Fluorescent dyes; Glass; Hybridisation; Inhibition kinetics; Isocyanates; Macrogols; Microcontact printing; Oligomers; Oligonucleotide; Polyethylene glycols; Polymers; Proteins; Solutions; Surface treatment; Ultrathin films
Publisher: American Chemical Society
ISSN: 1525-7797
Publisher URL: http://dx.doi.org/10.1021/bm0500695
Peer reviewed