Please use this identifier to cite or link to this item: http://hdl.handle.net/1959.3/216086

Title

A novel star PEG-derived surface coating for specific cell adhesion

Author(s)

Groll, Juergen;  Fiedler, Joerg;  Engelhard, Erika;  Ameringer, Thomas;  Tugulu, Stefano;  Klok, Harm-Anton;  Brenner, Rolf E.;  Moeller, Martin

Abstract

In this study a novel approach for the coating and functionalization of substrates for cell culture and tissue engineering is presented. Glass, silicon, and titanium panes were coated with an ultrathin film (30 +/- 5 nm) of reactive star-shaped poly(ethylene glycol) prepolymers (Star PEG). Homogeneity of the films was checked by optical microscopy and scanning force microscopy. These coatings prevent unspecific protein adsorption as monitored by fluorescence microscopy and ellipsometry. In order to allow specific cell adhesion the films were modified with linear RGD peptides (gRGDsc) in different concentrations. After sterilization, fibroblast, SaOS, and human mesenchymal stem cells (hMSC) were seeded on these substrates. Cell adhesion, spreading, and survival was observed for up to 30 days on linear RGD peptide (gRGDsc)-modified coatings, whereas no cell adhesion could be detected on unmodified Star PEG layers. By variation of the RGD concentration within the film the amount of cells that became adhesive could be controlled. When differentiation conditions are used for cultivation of hMSCs the cells show the expression of osteogenic marker genes after 14 days which is comparable to cultivation on cell culture plastic. Thus, the Star PEG/RGD film did not negatively influence the differentiation process. The high flexibility of the system considering the incorporation of biologically active compounds opens a broad field of future experiments.

Publication type

Journal article

Source

Journal of Biomedical Materials Research: Part A, Vol. 74, no. 4 (Sep 2005), pp. 607-617

Publication year

2005

FOR Code(s)

06 Biological Sciences;  09 Engineering

Keyword(s)

Adsorption;  Arginylglycylaspartic acid;  Biocompatible materials;  Cell adhesion;  Cell culture;  Cell differentiation;  Fibroblasts;  Fluorescence microscopy;  Macrogols;  Mesenchymal stem cells;  Osteoblast;  Polyethylene glycols;  Polymers;  Reverse transcription polymerase chain reaction;  RGD;  RNA;  Scanning force microscopy;  Silicon;  Substrates;  Surface modification;  Surface properties;  Surface treatment;  Thin films;  Tissue engineering;  Titanium

Publisher

John Wiley & Sons

ISSN

0021-9304

Publisher URL

http://dx.doi.org/10.1002/jbm.a.30335

Copyright

Copyright © 2005 Wiley Periodicals, Inc.

Peer reviewed